Apart from using different types of #PCR you can also play around the generic PCR cycle with some #PCRenhancers, just how you add #eggwhites in your cocktail (!)
What went wrong?
Low yield of PCR products
No amplification from #GCrichtemplates
Non-specific products
Presence of #PCRinhibitors
Solutions?
TABLE: Names, roles and doses of some #PCRenhancers.
Apart from the established chemicals like #DMSO, #Betaine, #Formamide, #BSA, #Trehalose, #T4gene32protein, #Carnitine, #NP40, #TritonX100, #Tween20, #PEG, #Gelatine, #Glycerol, #TMAC, #DTT etc., certain others like #deoxyinosine, #ethyleneglycol, #glycine are also reported to enhance PCR.
Hope it helps!
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