Fetal Bovine/ calf Serum is one of the most used growth supplement in #mammalian cell culture. But a lot of regulatory and manufacturing constraints are pushing the industry towards #synthetic and defined #supplements. Lets get to the petridish!
Why not FBS/FCS?
Batch-to-batch variability in quality and composition
It carries the risk of #anaphylactoid reactions, #xenoimmunization and zoonotic transmission when used for expanding cells destined for therapeutic purposes.
Increasing ethical concerns on grounds of animal cruelty
Increasing demand causing supply chain issues
High price plaguing basic research
What are alternatives to FBS/FCS?
Chemically-Defined Xeno-free Medium
Human Platelet Lysate XF (#hPL, Life Sc. production)
ITS liquid supplement (Insulin-transferrin-sodium selenite, #Sigma Aldrich)
SITE (ITS + Ethanolamine, #Sigma Aldrich)
ITS/SITE (Sigma Aldrich) + linoleic acid/oleic acid/BSA
Bovine Pituitary Extract (#BPE)
Earthworm Coelomic Fluid (CF)
Defined/ characterized FBS (#Hyclone)
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