#PCR product purification is as important and tricky as adding egg whites into your #WhiskeySour. Nevertheless all the downstream applications of the PCR product depend largely on the quality of the purified #DNA. Linear #acrylamide can serve as a very good enhancer here.
Protocol
The Sample DNA needs to be in a salt solution, or you may add 3M #SodiumAcetate pH 5.2 to it 1/10th the sample volume.
Add 10-20ug/mL of linear #Acrylamide to the sample.
Add one volume of #Isopropanol (preferably) or two and half volume of #Ethanol.
Mix and keep in -20C freezer for at least 15 mins (overnight would be best though!).
Centrifuge at ~12000g at 4C for 10 minutes.
Discard the supernatant and wash the visible pellet with 70% #Ethanol.
Repeat centrifugation, dry the pellet and dissolve in suitable buffer or water.
Merits
Linear #acrylamide can improve yield of >20bp PCR products, excluding most #oligos and #dNTPs.
Being an artificial chemical it does not have chances of being contaminated with other #NucleicAcids or #nucleases as is the case with other popular choices like #YeastRNA, #tRNA and #Glycerol.
#Acrylamide does not interfere with the A260/280 ratio, although #Glycerol might.
#Acrylamide does not interfere with DNA during phosphorylation with polynucleotide kinase like #tRNA or with DNA-protein interaction experiments like #Glycerol.
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